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エルゴチオネイン（ ERGO） による抗酸化作用と 神経細胞の分化の増強作用
https://hju.repo.nii.ac.jp/records/2000220
https://hju.repo.nii.ac.jp/records/20002205f2e7c5c-a352-4fa8-bdce-e811abe4fbf5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ronsyu73tsuchiya.pdf (3 MB)
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| アイテムタイプ | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
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| 公開日 | 2026-03-26 | |||||||||||
| タイトル | ||||||||||||
| タイトル | エルゴチオネイン（ ERGO） による抗酸化作用と 神経細胞の分化の増強作用 | |||||||||||
| 言語 | ja | |||||||||||
| タイトル | ||||||||||||
| タイトル | Ergothioneine (ERGO) Modulates Redox Balance and Promotes Neuronal Differentiation | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | Ergothioneine | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | oxidative stress | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | MAPK signaling | |||||||||||
| キーワード | ||||||||||||
| 言語 | en | |||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | neuronal differentiation | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 著者 |
土谷, 佳弘
× 土谷, 佳弘
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Ergothioneine (ERGO) is a naturally occurring thiol-containing amino acid derived from histidine and obtained exclusively through dietary intake, particularly from mushrooms. ERGO has been identified as a potent antioxidant and cytoprotective molecule that accumulates in mammalian tissues via the organic cation transporter OCTN1 (SLC22A4). In this study, we investigated two major biological effects of ERGO using rat adrenal pheochromocytoma PC12 cells: (1) its protective effect against hydrogen peroxide (H₂O₂)-induced oxidative stress and (2) its regulatory effect on neuronal differentiation induced by nerve growth factor (NGF). Cell viability and cytotoxicity were assessed by MTT and LDH assays, respectively. Western blot analysis revealed that ERGO suppressed oxidative stress-induced phosphorylation of MAPKs, including p38 and JNK. Furthermore, ERGO enhanced NGF-induced neurite outgrowth in a dose-dependent manner. These findings suggest that ERGO functions not only as an antioxidant but also as a modulator of neuronal differentiation signaling, potentially via MAPK and mTOR–S6K pathways. Cell proliferation was evaluated using the MTT assay. As expected, oxidative stress induced by H₂O₂ inhibited cellular growth. However, when H₂O₂ was administered alongside ERGO, this growth inhibition was alleviated. This observation suggests that ERGO promotes cellular proliferation in the presence of H₂O₂, and that is likely due to its antioxidant properties. The effect of ERGO on the MAPK pathway activated by oxidative stress H₂O₂ increased the activity of stress-related proteins, p38 and JNK. In contrast, the addition of ERGO reduced the activity of these proteins. This indicates that ERGO decreases reactive oxygen species and inhibits the activity of stress-related proteins. The effect of ERGO on NGF-induced neuronal differentiation NGF induced the differentiation of PC-12 cells into neuron-like structures, characterized by elongated extensions. The addition of ERGO further promoted the growth of these extensions in a dose-dependent manner. This implies that ERGO facilitates neuronal growth by augmenting NGF signaling. |
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| 言語 | en | |||||||||||
| 書誌情報 |
ja : 広島女学院大学論集 en : Bulletin of Hiroshima Jogakuin University 巻 73, p. 55-65, 発行日 2026-03-03 |
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| 出版者 | ||||||||||||
| 出版者 | 広島女学院大学 | |||||||||||
| 言語 | ja | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | PISSN | |||||||||||
| 収録物識別子 | 0374-8057 | |||||||||||
| 書誌レコードID | ||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||
| 収録物識別子 | AN1021267X | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
