| Item type |
紀要論文 / Departmental Bulletin Paper(1) |
| 公開日 |
2023-03-03 |
| タイトル |
|
|
タイトル |
鶏卵アレルギー患者血清を用いた卵黄のアレルゲン性に関する研究 |
|
言語 |
ja |
| タイトル |
|
|
タイトル |
Determination of Allergenic Proteins in Hen's Egg Yolk Using Immunoblot Analysis with Sera From Chicken Egg Allergy Patients |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
アレルギー |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
鶏卵 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
血清 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
アレルゲン姓 |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
departmental bulletin paper |
| 著者 |
坂井, 堅太郎
水羽, 陽子
朝日(清水池), 綾子
鉄穴森, 陽子
谷廣, 佳奈子
高松, 寛子
山内, 真知子
太田, 喜子
難波, 麻紀
高見澤, 怜子
岡本, 直子
篠田, 有希子
渡邉, 慶子
Sakai, Kentaro
Mizuha, Yoko
Shimizuike-Asahi, Ayako
Kanamori, Yoko
Tanihiro, Kanako
Takamatsu, Hiroko
Yamauchi, Machiko
Ohta, Yoshiko
NANBA, Maki
TAKAMISAWA, Reiko
OKAMOTO, Naoko
SHINODA, Yukiko
Watanabe, Keiko
美野, 祐里佳
Mino, Yurika
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Chicken egg allergy is frequently observed in infants, some of who therefore have to avoid hen's egg from daily diet to eliminate unfavorably allergenic symptoms. Hen's egg is composed of two soluble parts; one is egg white which has been characterized as major in allergenic portion, while the other is egg yolk which is estimated as miner in that, regardless of less accumulation of research in allergenicity of egg yolk proteins.
So that using the method of immunoblot analysis, IgE-binding capacity against chicken egg proteins in egg yolk as well as egg white was determined in ten sera from infantile patients with chicken egg allergy. In egg white proteins, all of the serum IgE recognized ovalbumin, and two of that bound to ovomucoid. While in egg yolk proteins, serum IgE from one patient but not the others clearly bound to the protein at the molecular weight weight of 35 kD. This protein was also detectable as an aooarent band on SDS-polyacrylamide gel electrophoresis stained by Coomassie Brilliant Blue R-250 and estimated to be phosvitin, which is a hydeophobic apolipoprotein originally synthesized by the liver, translated to the ovary through the blood stream, and then packaged into egg yolk.
These results raise that the general tendency of strong allegenicity against patients suffering from chicken egg allergy is in egg white rather than in egg yolk, and suggest that phosvitin si a candidate for noticeably allergenic molecule in egg yolk. |
| 書誌情報 |
広島女学院大学論集
巻 58,
p. 203-209,
発行日 2008-12-20
|
| 出版者 |
|
|
出版者 |
広島女学院大学 |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
03748057 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
| 著者版フラグ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
ronsyu58sakai.pdf (648.7 kB)
