WEKO3
アイテム
藻類の回定方法についての検討：Undaria pinnatifidaの電子顕微鏡的観察
https://hju.repo.nii.ac.jp/records/964
https://hju.repo.nii.ac.jp/records/964d2d4f929-2cdd-41c3-995f-c34e91d05085
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ronsyu30okuda.pdf (1.5 MB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2023-03-03 | |||||||||||
| タイトル | ||||||||||||
| タイトル | 藻類の回定方法についての検討：Undaria pinnatifidaの電子顕微鏡的観察 | |||||||||||
| 言語 | ja | |||||||||||
| タイトル | ||||||||||||
| タイトル | Study on Fixation Method of Aｌgae：Electron microscopic observation of Undaria pinnatifida | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | jpn | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 藻類 | |||||||||||
| キーワード | ||||||||||||
| 主題Scheme | Other | |||||||||||
| 主題 | 回定方法 | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||||
| 著者 |
奥田, 弘枝
× 奥田, 弘枝
× Okuda, Hiroe
|
|||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | There is a large number of algae containing much water and mucoid substance in there bodies. As they thrive in sea water，a re susceptible to the osmolarity of fixative solution, and it is said that fixation of the various structures in their cells are difficult. This report, seeking the optimum fixation method to observe in details and correctly the form of the tissue，organella and the cell of Undaria pinnatifida，3 kinds of fixation methods，viz. single fixation of osmic acid employed since olden times，mixed fixation of osmic acid and glutaraldehyde，and double fixation of prefixation by glutaraldehyde and postfixation by osmic acid have been tried，compared and studied. Materials are healthy lamina of Undaria pinnatifida collected on the coast of Onomichi in April，1978. After fixing the material with the respective fixing solution，alcohol dehydration，and after embedding with epoxy resins making them in ultra thin sections，double staining by Millonig solution and uranyl acetate solution，has been applied，and observed with electron microscopes (HS-7 type and JEM 100-B type).Findings were that single fixation by osmic acid proved effective for observation of nucleus. nucleolus，vacuole, vesicle，ground substance and cell wall，while unfit for observation of chloroplast, mitochondria and Golgi body. With mixed fixation of osmic acid and glutaraldehyde，vacuole，ground substance and cell wall can be observed very well. however，on other organella，electron density was low, and the structure is obscure. It can be said that with glutaraldehyde pre-fixation，osmic acid post-fixation, fixation effect and electron staining effect of every organella was good showing that this is the most optimum fixation method. |
|||||||||||
| 書誌情報 |
広島女学院大学論集(第二部) 巻 30, p. 185-198, 発行日 1980-12-20 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | 広島女学院大学 | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0374-8057 | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
